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Image Search Results
Journal: The Kaohsiung journal of medical sciences
Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.
doi: 10.1002/kjm2.12370
Figure Lengend Snippet: FIGURE 1 MiR-152-5p was downregulated and bromodomain containing 4 (BRD4) was upregulated in cervical carcinoma tissue and adjacent normal tissues. (A) The expression of miR-152-5p was detected by quantitative real-time PCR (qRT-PCR) in cervical carcinoma tissue and adjacent normal tissues. (B) The expression of BRD4 was detected by qRT-PCR. (C) The correlation was analyzed by Pearson's analysis. ***p < 0.001
Article Snippet: For the detection of
Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR
Journal: The Kaohsiung journal of medical sciences
Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.
doi: 10.1002/kjm2.12370
Figure Lengend Snippet: FIGURE 2 Apigenin inhibited the proliferation and invasion of cervical carcinoma cells by regulating miR-152-5p expression. (A) Quantitative real-time PCR (qRT-PCR) was conducted to determine the expression of miR-152-5p and bromodomain containing 4 (BRD4) in cell lines of HOSEpiC, C33A, Hela, SiHa, and CaSki. (B) qRT-PCR was used to detect the transfection efficiency of miR-152-5p in Hela and CaSki cells transfected with miR- 152-5p inhibitor and the NC inhibitor. (C) MTT was conducted to evaluate the proliferation in Hela and CaSki cells treated with apigenin, cells treated with apigenin and transfected with miR-152-5p inhibitor, cells treated with apigenin and transfected with NC, and negative control, respectively. (D) Transwell invasion assay was applied to determine the cell invasion. ***p < 0.001
Article Snippet: For the detection of
Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Transwell Invasion Assay
Journal: The Kaohsiung journal of medical sciences
Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.
doi: 10.1002/kjm2.12370
Figure Lengend Snippet: FIGURE 3 Apigenin inhibited epithelial–mesenchymal transition (EMT) of cervical carcinoma cells by regulating miR- 152-5p expression. (A) Quantitative real- time PCR (qRT-PCR) was used to calculate the expression of miR-152-5p and bromodomain containing 4 (BRD4). (B) Western blotting was conducted to detect the protein level of BRD4, E- cadherin, N-cadherin, and MMP9. ***p < 0.001
Article Snippet: For the detection of
Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot
Journal: The Kaohsiung journal of medical sciences
Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.
doi: 10.1002/kjm2.12370
Figure Lengend Snippet: FIGURE 4 Apigenin suppressed the proliferation and invasion of Hela and CaSki cells through regulating bromodomain containing 4 (BRD4) expression. (A) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of BRD4 in Hela and CaSki cells transfected with pcDNA3.1-BRD4 and the negative control (NC) control. (B) MTT was conducted to evaluate the proliferation of Hela and CaSki cells treated with apigenin, cells treated with apigenin and transfected with pcDNA3.1-BRD4, cells treated with apigenin and transfected with NC, and negative control, respectively. (C) Transwell was applied to determine the cell invasion. ***p < 0.001
Article Snippet: For the detection of
Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Control
Journal: The Kaohsiung journal of medical sciences
Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.
doi: 10.1002/kjm2.12370
Figure Lengend Snippet: FIGURE 5 Apigenin inhibited epithelial–mesenchymal transition (EMT) of Hela and CaSki cells through regulating bromodomain containing 4 (BRD4) expression. (A) Quantitative real-time PCR (qRT-PCR) was used to calculate the expression of BRD4. (B) Western blotting was conducted to detect the protein level of BRD4, E-cadherin, N- cadherin, and MMP9. ***p < 0.001
Article Snippet: For the detection of
Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot
Journal: The Kaohsiung journal of medical sciences
Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.
doi: 10.1002/kjm2.12370
Figure Lengend Snippet: FIGURE 6 miR-152-5p could directly bind to bromodomain containing 4 (BRD4) in Hela and CaSki cells. (a) The binding site of miR-152-5p with BRD4 was predicted according to TargetScan (http://www. targetscan.org/).(B) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of miR-152-5p in Hela and CaSki cells transfected with miR-152-5p mimics and the negative control (NC) mimics. (C) Targeting relationship between miR-152-5p and BRD4 was identified by luciferase reporter assay. ***p < 0.001
Article Snippet: For the detection of
Techniques: Binding Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Luciferase, Reporter Assay
Journal: The Kaohsiung journal of medical sciences
Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.
doi: 10.1002/kjm2.12370
Figure Lengend Snippet: FIGURE 7 Apigenin suppressed proliferation and invasion by regulating miR-152-5p/bromodomain containing 4 (BRD4) axis in Hela and CaSki cells. (A) Quantitative real-time PCR (qRT-PCR) was used to detect the transfection efficiency of BRD4 in Hela and CaSki cells transfected with si-BRD4 and the negative control (NC) control. (B) MTT was conducted to determine the proliferation of cells treated with apigenin and transfected with miR-152-5p inhibitor, cells treated with apigenin and co-transfected with miR-152-5p inhibitor and si-NC, cells treated with apigenin and co-transfected with miR-152-5p inhibitor and si-BRD4, and the control. (C) Transwell invasion assay was performed to evaluate the cell invasion. ***p < 0.001
Article Snippet: For the detection of
Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Negative Control, Control, Transwell Invasion Assay
Journal: The Kaohsiung journal of medical sciences
Article Title: Apigenin suppresses proliferation, invasion, and epithelial-mesenchymal transition of cervical carcinoma cells by regulation of miR-152/BRD4 axis.
doi: 10.1002/kjm2.12370
Figure Lengend Snippet: FIGURE 8 Apigenin suppressed epithelial–mesenchymal transition (EMT) by regulating miR-152-5p/bromodomain containing 4 (BRD4) axis in Hela and CaSki cells. (A) Quantitative real-time PCR (qRT-PCR) was used to calculate the expression of BRD4. (B) Western blotting was conducted to detect the protein level of BRD4, E-cadherin, N-cadherin, and MMP9. ***p < 0.001
Article Snippet: For the detection of
Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot